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Chymosin B

  1. Precision Fermentation
  2. Proteins
  3. Enzyme
  4. Chymosin B

    Technical Data

    Type of Protein Enzyme
    Protein name Chymosin B
    Companies producing this protein
    • Danisco
    • DSM
    • Chr. Hansen (part of Novonesis)
    Function (dairy alternative, colorant, texture, taste...) Coagulation of milk (Barros et al., 2025)
    Target application (cheese, infant formula, meat...) Cheese making (Vallejo et al., 2012)
    Purity requirement/purification values Has been purified up to 98% (Fährenmark et al., 2002)
    Techno-functional properties Cleavage of κ-caseins in milk make for milk clotting (Chitpinityol & Crabbe, 1998)
    Nutritional properties No real nutritional properties itself, the main nutritional properties come from the coagulation of milk (see target application).
    Advantages Chymosin specifically targets milk caseins and exhibits low proteolytic activity for other proteins, enabling high yields in cheese production (Barros et al., 2025).
    Challenges/risk factors
    • Loss in activity at a pH lower than 4 and higher than pH 9.8. Also rapid loss in activity at temperatures above 55°C (Chitpinityol & Crabbe, 1998)
    • Even after heat-inactivation, some residual activity could still be present in the cheese, leading to extra, unintended proteolysis during ripening (Hayes et al., 2002).
    Aspergillus niger
    Escherichia coli
    Kluyveromyces lactis
    Lactococcus lactis
    Trichoderma reesei

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